The 24 volt wire is the one you want interrupted with the eStop. The system stays grounded together and power is removed from the grblShield and the motors.
Warning: If you interrupt the ground line with the eStop the motor current will find it's way back though the USB port to the computer. This is not good on multiple levels. (1) USB is not meant to carry that much current. (2) the power supply ground and the computer ground (the USB ground) may be at different potentials. This risks blowing out any of the PC's USB port, the Arduino's USB port, the TI driver chips, the power supply itself.
Please note in the diagram the "hot" wire is red. The actual wire that came with the kit in Batch #1 is black. We will be updating this to a red wire in the future.
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